Welcome to the BIDMC Mass Spectrometry Facility and John Asara Laboratory
'Specializing in a multi-omics proteomics, metabolomics & lipidomics approach'
ABOUT US: The mass spectrometry, proteomics, metabolomics and lipidomics facility at Beth Israel Deaconess Medical Center was established in 2004 led by Dr. John Asara and continues to grow rapidly. We provide excellent proteomics, metabolomics and lipidomics services with low prices and fast turnaround times.
We have cutting edge instruments operated at optimal sensitivity and work and publish with world class researchers. We also maintain an active internal cancer proteomics and small molecule research program. Our expertise is in identifying and quantifying protein modifications and dynamic protein-protein interactions in addition to polar metabolomics profiling, 13C/15N metabolic flux analysis and lipidomics profiling. We accept collaborations from anywhere in the world and look forward to helping you and progressing your research to the next level.
New core flier......click for downloadable version
New core flier......click for downloadable version
Post-doc/Sr Researcher position available in the lab in Multi-Omics
NEWS:
NEWS:
- We recently published a Nature Protocol article on our method for performing 13C/15N metabolic flux via polarity switching and SRM click here
- Coming soon: a new Thermo Fusion Lumos Tribrid mass spectrometer
- Our AB/SCIEX 6500 QTRAP for expanded metabolomics and 13C/15N flux work is now installed and running....
- We upgraded our proteomics/phosphoproteomics platform with an ultra fast and ultra sensitive Thermo QExactive HFx via the BIDMC Capital Equipment Award (one of Thermo's fastest and most sensitive instruments)
- Our Lipidomics profiling platform is now operating on the Thermo QExactive Plus/HF Orbitrap with LipidSearch and Elements software
Proteomics, Metabolomics & Lipidomics
- Protein Complex Identification
(protein complexes, protein-protein interactions, single protein ID) - Identification of Post-Translational Modifications
(phosphorylation, acetylation, ubiquitination, etc.) - Relative Protein Quantification
(MS1 based label-free quantification, SILAC, Avg. TIC, TMT, etc.)- Protein complex stoichiometry
- Quantification of phosphorylation sites
- PPI networks
- Quantitative Metabolomics Profiling
(endogenous polar metabolites from any cell or tissue source)- Steady-state profiling
- C13/N15 metabolic flux analysis
- Untargeted metabolomics
- Lipidomics Non-targeted Profiling
(High resolution; more than 66 sub-classes of lipids identified and quantified)- C13 lipidomic flux coming soon
Instrumentation
- Thermo Scientific Fusion Lumos Tribrid hybrid Orbitrap - soon
- Thermo Scientific QExactive HFx Orbitrap (ultra fast and ultra sensitive for proteomics, untargeted metabolomics, etc.)
- Thermo Scientific QExactive Plus Orbitrap (high resolution, extremely fast scan speeds, HCD fragmentation, quantitative)
- Thermo Scientific Elite Orbitrap (ultra-high resolution and fast scan speed, extremely sensitive, CID and HCD fragmentation)
- AB/SCIEX 6500 QTRAP hybrid triple quadrupole
- AB/SCIEX 5500 QTRAP triple quadrupole
(Metabolomics profiling, targeted quantification) - Thermo Scientific EASY-nLC1200 UPLC
- Proxeon EASY-nLC II (3)
(nanoliter flow rates, very reproducible and robust) - Shimadzu Prominence PDA-UFLC (2)
- Agilent 1100 and 1200 HPLCs
AB/SCIEX 6500 QTRAP
Thermo (Proxeon) EASY-nLC II (3)
AB/SCIEX 5500 QTRAP
